Image early endosomes and vesicle trafficking in live cells with CellLight™ Early Endosomes-GFP. This ready-to-use fluorescent protein-based reagent contains a Rab5a targeting sequence fused to emerald GFP (emGFP) and utilizes the powerful BacMam technology for delivery. To use, simply add the reagent to your cells, incubate overnight for protein expression then visualize. Unlike expression vectors, BacMam reagents enable titratable and reproducible expression and offer high co-transduction efficiency, therefore multiple BacMam reagents can readily be used in the same cell. Recent improvements made to the BacMam system (BacMam version 2.0) enable efficient transduction in a wider variety of cells including primary neurons and stem cells. The reagent also tolerates fixation with formaldehyde and thus is compatible with fixed-cell analysis, which is required for multiplex experiments using antibodies.
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Store at 2-6°C, protected from light. Do Not Freeze.
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