Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.
Product Information
Format
Purified
Control
Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.
Presentation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.
Applications
Application
This Anti-methyl-PP2A Antibody, C subunit, clone 2A10 is validated for use in WB for the detection of methyl-PP2A.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated, synthetic peptide (C-βA-RRTPDYFL-Ome) corresponding to amino acids 302-309 of human PP2A catalytic subunit. Clone 2A10.
Clone
2A10
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes the methylated form of PP2A, catalytic subunit.
Isotype
IgG1κ
Species Reactivity
Drosophila Human Mouse Yeast (S. cerevisiae) Rat Pig Rabbit Chicken
Species Reactivity Note
Human, mouse and yeast. Predicted to cross react with rat, pig, rabbit, chicken and Drosophila.
Evaluated by western blot on lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.
Western Blot Analysis: 1-5 µg/mL of this antibody detected the methylated form of PP2A catalytic subunit in yeast lysates lacking the methylestrase (increased methylation). Treatment of the blot with 0.2 M NaOH will demethylate proteins, thus eliminating detection of PP2A.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Packaging Information
Material Size
150 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
Dr, H, M, Yeast (S. cerevisiae), R, Po, Rb, Ch
WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-1479
Replaces
05-546
Description
Anti-methyl-PP2A Antibody, C subunit, clone 2A10
Background Information
Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.
Product Information
Format
Purified
Control
Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.
Presentation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.
Applications
Application
This Anti-methyl-PP2A Antibody, C subunit, clone 2A10 is validated for use in WB for the detection of methyl-PP2A.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated, synthetic peptide (C-βA-RRTPDYFL-Ome) corresponding to amino acids 302-309 of human PP2A catalytic subunit. Clone 2A10.
Clone
2A10
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes the methylated form of PP2A, catalytic subunit.
Isotype
IgG1κ
Species Reactivity
Drosophila Human Mouse Yeast (S. cerevisiae) Rat Pig Rabbit Chicken
Species Reactivity Note
Human, mouse and yeast. Predicted to cross react with rat, pig, rabbit, chicken and Drosophila.
Evaluated by western blot on lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.
Western Blot Analysis: 1-5 µg/mL of this antibody detected the methylated form of PP2A catalytic subunit in yeast lysates lacking the methylestrase (increased methylation). Treatment of the blot with 0.2 M NaOH will demethylate proteins, thus eliminating detection of PP2A.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
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