Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins.
Product Information
Format
Culture Supernatant
Control
C6 cell lysate
Presentation
Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
Flow Cytometry
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected N-cadherin in human cervical carcinoma tissue.
Immunocytochemistry Analysis: 1:100-250 dilution from a previous lot detected N-cadherin in HeLa cell lysate.
Immunoprecipitation: 1:40 dilution from a previous lot was used on IP.
Flow Cytometry: 1:30 dilution from a previous lot was used on FC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of human N-cadherin.
FUNCTION:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. SUBUNIT STRUCTURE:Interacts with CDCP1. SUBCELLULAR LOCATION:Cell membrane; Single-pass type I membrane protein. SEQUENCE SIMILARITIES:Contains 5 cadherin domains.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on C6 cell lysate.
Western Blot Analysis: 1:50,000 dilution of this antibody was used to detect N-cadherin in C6 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins.
Product Information
Format
Culture Supernatant
Control
C6 cell lysate
Presentation
Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
Flow Cytometry
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected N-cadherin in human cervical carcinoma tissue.
Immunocytochemistry Analysis: 1:100-250 dilution from a previous lot detected N-cadherin in HeLa cell lysate.
Immunoprecipitation: 1:40 dilution from a previous lot was used on IP.
Flow Cytometry: 1:30 dilution from a previous lot was used on FC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of human N-cadherin.
FUNCTION:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. SUBUNIT STRUCTURE:Interacts with CDCP1. SUBCELLULAR LOCATION:Cell membrane; Single-pass type I membrane protein. SEQUENCE SIMILARITIES:Contains 5 cadherin domains.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on C6 cell lysate.
Western Blot Analysis: 1:50,000 dilution of this antibody was used to detect N-cadherin in C6 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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