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FISH TAG DNA RED KIT WITH ALE 1 KIT

 
包装:
运保温度:
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其它组分:

Alexa Fluor® reactive dye【包装:5 vials ineach of 2pouches,,运保温度:≤–20˚C;desiccate;protect from light】
Dimethylsulfoxide (DMSO)【包装:200 μL,,运保温度:≤–20˚C;desiccate】
Dithiothreitol (DTT)【包装:100 μL,,运保温度:≤–20˚C】
Glycogen, ultrapure, nulcease free【包装:20 μL,,运保温度:≤–20˚C】
Sodium bicarbonate【包装:84 mg,,运保温度:≤–20˚C】
Water, nuclease free【包装:4.0 mL,,运保温度:≤–20˚C】
DNA nucleotide mix【包装:80 μL,,运保温度:≤–20˚C】
DNA polymerase I【包装:25 μL,,运保温度:≤–20˚C】
DNase I【包装:15 μL,,运保温度:≤–20˚C】
Nick translation buff er【包装:250 μL,,运保温度:≤–20˚C】
SlowFade® Gold antifade reagent【包装:2 mL,,运保温度:≤–20˚C; protect from light】
Binding buff er【包装:6.0 mL,,运保温度:≤25˚C】
Wash buff er【包装:3.2 mL,,运保温度:≤25˚C】
Elution buff er【包装:6.0 mL,,运保温度:≤25˚C】
Spin columns and collection tubes【包装:20 columnswith tubes,,运保温度:≤25˚C】
Collection tubes【包装:20 tubes,,运保温度:≤25˚C】


描述:

FISH Tag™ DNA kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. FISH Tag™ DNA kits employ a two-step labeling technology, using nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. [PureLink] nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent for excellent photostability during imaging. FISH Tag™ DNA kits are ideal for labeling DNA targets in chromosomal spreads, and for in situ analysis of mitochondrial DNA localization and content. The FISH Tag™ DNA Red Kit includes red-fluorescent Alexa Fluor® 594 dye (excitation/emission maxima ~590/615) for exceptional signal intensity. FISH Tag™ DNA kits are available in single-color kits containing one of four Alexa Fluor® dyes or in a multicolor kit that contains all four dyes.


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