Recombinant protein corresponding to amino acids 352–605 of human Nrf2
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Immunocytochemistry (ICC)
1-2 ug/ml
Immunofluorescence (IF)
1-2 ug/ml
Western Blot (WB)
0.5 -2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Nrf2 (nuclear factor erythroid 2-related factor 2) is a transcription activator which plays a key role in protecting the cell from oxidative stress and inflammation. It is primarily localized in the cytoplasm under normal conditions, but under conditions of oxidative stress, Nrf2 migrates to the nucleus to upregulate expression of genes with ARE (antioxidant response elements) in the promoter region. The upregulated genes include those involved in cellular protection, drug transport, detoxification, and anti-oxidant defense. The protein is ubiquitously expressed in various tissues, with the highest levels found in kidney, muscle, lung, heart, liver, and brain.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.