Peptide corresponding to amino acids 26–38 of human Granzyme B
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
1 ug/ml X 10^6 cells
Western Blot (WB)
1-2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Granzyme B is a serine protease contained within cytoplasmic granules produced by cytotoxic T lymphocytes and natural killer cells. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. It can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzyme B has also been shown to play an important role in activation induced cell death of T helper 2 cells.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
