PathScan? Phospho-DDR1 (panTyr) Sandwich ELISA Kit

• 产品编号：CST-7863C      品牌：CST       原厂货号：7863C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit用于检测人细胞中内源性酪氨酸位点被磷酸化的DDR1蛋白的表达（如图1）。图2显示试剂盒的灵敏度。内部检测显示，本试剂盒能检测列举出的物种蛋白，但不排除用于其它物种同源蛋白检测的可能。PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit利用固相双抗夹心酶联免疫检测法对内源性酪氨酸位点被磷酸化的DDR1蛋白进行检测。微孔中已经包被了抗DDR1的兔源单克隆抗体，与细胞裂解液共孵育后，磷酸化的和未磷酸化的DDR1均能被包被抗体捕获。多次洗板之后，加入抗酪氨酸磷酸化的DDR1的抗体对被捕获的酪氨酸位点被磷酸化的DDR1蛋白进行检测，然后加入HRP标记的抗小鼠的IgG对检测抗体进行识别，最后加入HRP的底物TMB进行显色反应。按照相对光单位，发射光强度与酪氨酸位点被磷酸化的DDR1蛋白量成正比。巨噬细胞集落刺激因子（M-CSF, CSF-1）受体是由原癌基因c-fms编码的一种膜嵌合的酪氨酸激酶。M-CSF 受体表达在单核细胞（巨噬细胞及其前体细胞）上并促进这一系列血细胞的生长和发育(1-3)。M-CSF和它的受体结合后会引起受体二聚化、激活、以及细胞质内作为含SH2信号蛋白结合位点的酪氨酸残基自磷酸化(4)。至少有5个主要的酪氨酸自磷酸化位点。Tyr723 (小鼠Tyr721)位于激酶插入（KI）区。磷酸化的Tyr723能够与PI3K激酶的p85亚基以及PLC-γ 2结合(5)。磷酸化的Tyr809能够为Shc提供停泊位点（5）。该受体的过度激活会导致各种细胞体系呈现出恶性特征（6）。激活的M-CSF 受体表明晚期上皮样卵巢癌（7）和乳腺癌预后不良（8）。

1. Vogel, W. (1999) FASEB J 13 Suppl, S77-82.
2. Ferri, N. et al. (2004) Am J Pathol 164, 1575-85.

原厂资料：

Specificity / Sensitivity

PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit detects endogenous levels of tyrosine-phosphorylated DDR1 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Phospho-DDR1 (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine-phosphorylated DDR1 protein. A DDR1 rabbit antibody has been coated on the microwells. After incubation with cell lysates, DDR1 protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a phospho-tyrosine mouse mAb is added to detect captured tyrosine-phosphorylated DDR1 protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of DDR1 protein phosphorylated on tyrosine residues.

Background

The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Aberrant expression and signaling of DDRs have been implicated in human disease related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1).

1. Vogel, W. (1999) FASEB J 13 Suppl, S77-82.
2. Ferri, N. et al. (2004) Am J Pathol 164, 1575-85.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).