PathScan? EMT Duplex IF Kit

产品编号：CST-7771S 品牌：CST 原厂货号：7771S
产品分类：ELISA及其它免疫分析 > ELISpot及FluoroSpot > FluoroSpot试剂盒
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包装：

运保温度:

–20°C

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其它组分：

PathScan® EMT Duplex IF Kit Primary Antibody Cocktail【包装：100 µl，，运保温度：–20°C】

PathScan® Duplex IF Kit Detection Cocktail I【包装：100 µl，，运保温度：–20°C】

描述：

Vimentin mAb能检测内源性水平的总波形蛋白。E-cadherin mAb能检测内源性水平的总上皮细胞钙粘蛋白，与相关的家族成员，如神经钙粘蛋白，没有交叉反应。单克隆抗体通过用合成肽免疫动物制备，该合成肽是人波形蛋白Arg45周围的残基或者是人上皮细胞钙粘蛋白Pro780周围的残基。The PathScan® EMT Duplex IF Kit能够通过手动的免疫荧光显微镜检查或者自动成像和激光扫描高通量平台，提供一种新的方法来同步监视上皮细胞或间质起源，以及那些进行中的上皮-间质转化。此试剂盒包含一个两种高品质抗体的混合物，该抗体主要针对波形蛋白和上皮细胞钙粘蛋白，以及使用Alexa Fluor®系列荧光染料的检测混合物。抗体和配对染料预先优化，每个试剂盒中的试剂能进行100次试验。（工作体积为100μl/试验）。上皮-间质转化（EMT）涉及到一个生物学的过程，在该过程中细胞经历了一系列生物化学变化，诱导了形态学的转变，从一个上皮的，极化的，黏附状态变为具有迁移能力的不规则的，长的，间质表型（1,2）。EMTs分为三种亚型：一种与移植，胚胎发育和器官发育有关；一种与炎症和纤维变性有关；还有一种与侵入和转移有关（1）。在这个转化过程中与细胞有关的分子变化分别包括上皮细胞钙粘蛋白的损失和波形蛋白表达的增加，标志上皮和间质标记（3-5）。为数众多的研究都证明EMT是癌症转移中重要的一步（5-7）。上皮细胞钙粘蛋白被认为是侵入和肿瘤形成的主动抑制剂（8）。针对细胞外的刺激，波形蛋白调节多条信号通路来诱导空间重组和结构改变（9），及在与侵入和转移有关的活动细胞中观察EMT表型（6）。

原厂资料：

Specificity / Sensitivity

Vimentin mAb detects endogenous levels of total vimentin protein. E-cadherin mAb detects endogenous levels of total E-cadherin protein and does not cross react with related family members such as N-cadherin.

Source / Purification

Monoclonal antibodies were produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg45 of human vimentin protein or residues surrounding Pro780 of human E-cadherin protein.

Description

The PathScan® EMT Duplex IF Kit offers a novel method to simultaneously monitor cells of epithelial or mesenchymal origin, as well as those undergoing an epithelial-mesenchymal transition (EMT) using manual immunofluorescence microscopy or automated imaging and laser scanning high content platforms. This kit contains a cocktail of two high quality primary antibodies targeted against vimentin and E-cadherin, as well as a detection cocktail utilizing the Alexa Fluor® series of fluorescent dyes. Antibody and dye pairings have been pre-optimized and each kit contains enough reagents for 100 assays (based on a working volume of 100 μl/test).

Background

Epithelial-mesenchymal transition (EMT) refers to a biological process in which cells undergo a series of biochemical changes that induce a morphological transformation from an epithelial, polarized, adhesive state to an irregular, elongated, mesenchymal phenotype that enables migratory capacity (1,2). EMTs are classified into three subtypes: those involved in implantation, embryogenesis, and organ development; those associated with inflammation and fibrosis; and those involved in invasion and metastasis (1). Molecular changes that are associated with cells during this transformation include the loss of E-cadherin and gain of vimentin expression, hallmark epithelial and mesenchymal markers, respectively (3-5). Numerous studies have established that EMT is an essential step in cancer metastasis (5-7). E-cadherin is regarded as an active suppressor of invasion and tumorigenesis (8). In response to extracellular stimuli, vimentin coordinates various signaling pathways to induce spatial reorganization and structural changes (9), reminiscent of the EMT phenotype observed in motile cells involved in invasion and metastasis (6).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.