PathScan? Total Fatty Acid Synthase Sandwich ELISA Kit

• 产品编号：CST-7689C      品牌：CST       原厂货号：7689C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

该试剂盒经过内部测试，可识别制定物种的蛋白质，也可能识别一些其它物种的同源性蛋白。The PathScan? Total Fatty Acid Synthase Sandwich ELISA Kit是一个固相夹心酶联免疫分析（ELISA）试剂盒，可以检测转染水平的全部脂肪酸合成蛋白（FASN）。FASN兔抗体已经被包被在微孔上。在和细胞裂解液孵育时，FASN将被包被抗体捕获。在接下来的洗涤后，生物素化的FASN兔检测抗体将结合上，以便识别捕获的FASN蛋白。HRP-连接的streptavidin将用来识别检测抗体。HRP底物TMB将用来显色。显色后的吸光程度正比于FASN的量。试剂盒中的抗体仅对该试剂盒特制。脂肪酸合成酶(FASN)催化乙酰辅酶A和丙二酰辅酶A合成长链脂肪酸。FASN以同源二聚体发挥作用，有7种不同的催化活性，可以在肝脏中制造脂质以便输送到有代谢活性的组织或脂肪储存组织。在大多数人类组织中，FASN都以最小量表达，因为它们需要循环利用脂肪酸以制造新的结构脂质（1）。最近发现，FASN表达增加似乎是人类恶性肿瘤中的一个常见表型。在乳腺癌中，免疫组织化学染色发现FASN的水平与乳腺癌体积直接相关（2）。研究还发现FASN在肺癌和前列腺癌中高表达，FASN的表达是乳腺癌和前列腺癌预后不良的指标（3-5）。另外，抑制FASN的表达对人类癌细胞有选择性的细胞毒作用（5）。因此，FASN吸引了越来越多的研究者兴趣，它除了在代谢综合征中发挥作用外，它可能同时蕴含着在肿瘤的诊断和治疗中的潜在作用（6,7）。

1. Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
2. Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40.
3. Kawamura, T. et al. (2005) Pathobiology 72, 233-240.
4. Shah, U.S. et al. (2006) Hum Pathol 37, 401-409.
5. Kuhajda, F.P. (2000) Nutrition 16, 202-8.
6. Tian, W.X. (2006) Curr Med Chem 13, 967-977.
7. Kusunoki, J. et al. (2006) Endocrine 29, 91-100.

原厂资料：

Specificity / Sensitivity

This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Total Fatty Acid Synthase Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of fatty acid synthase protein (FASN). A FASN Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, FASN is captured by the coated antibody. Following extensive washing, a biotinylated FASN Rabbit Detection Antibody is added to detect the captured FASN protein. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of FASN. 
 Antibodies in kit are custom formulations specific to kit.

Background

Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FASN is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FASN is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (1). 
 Recently, increased expression of FASN has emerged as a phenotype common to most human carcinomas. In breast cancer, immunohistochemical staining showed that the levels of FASN are directly related to the size of breast tumors (2). Studies also showed that FASN is highly expressed in lung and prostate cancers and that FASN expression is an indicator of poor prognosis in breast and prostate cancer (3-5). Furthermore, inhibition of FASN is selectively cytotoxic to human cancer cells (5). Thus, increased interest has focused on FASN as a potential target for the diagnosis and treatment of cancer as well as metabolic syndrome (6,7).

1. Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
2. Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40.
3. Kawamura, T. et al. (2005) Pathobiology 72, 233-240.
4. Shah, U.S. et al. (2006) Hum Pathol 37, 401-409.
5. Kuhajda, F.P. (2000) Nutrition 16, 202-8.
6. Tian, W.X. (2006) Curr Med Chem 13, 967-977.
7. Kusunoki, J. et al. (2006) Endocrine 29, 91-100.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).