Human Leptin/OB (hLeptin)

• 产品编号：CST-5719SC      品牌：CST       原厂货号：5719SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它细胞因子和生长因子
• 应用分类：

描述：

重组人瘦素（hLeptin）Val22-Cys167（Accession #NP_000221）由CST公司用大肠杆菌生产。重组hLeptin在氨基末端有Met，分子量为16,026。DTT还原和非还原的蛋白电泳可见15kDa的多肽。重组hLeptin的氨基末端MVPIQ使用氨基酸测序验证。6μg的还原（+）和非还原（-）的重组hLeptin经SDS-PAGE验证纯度>98%。 每孔纯度超过98%。重组hLeptin的生物活性使用在MCF7细胞中促进TNF-α产生的能力进行确定。产生此作用的最小浓度为50ug/ml。内毒素小于0.01ng/1μg hLeptin。有载体：冻干，PBS经0.22μm孔过滤，pH为7.2，每μg hLeptin含有20μg hLeptin。无载体：冻干，PBS经0.22μm孔过滤，pH值7.2。瘦素是一个16kDa的脂肪细胞源性的激素，可以传递机体能量状态到下丘脑，导致食欲减弱和调节能量消耗（1,2）。瘦素在免疫和生殖中作为多效性细胞因子发挥作用（1-4）。至少存在5种瘦素受体的亚型，但是只有OB-Rb可以胜任信号转导（1,2）。OB-Rb在很多细胞内表达，包括T细胞、B细胞、胶质细胞等（1-6）。瘦素/OB-Rb相互作用可诱导Jak2、PI3K、Erk1/2和Stat3的激活（1,2）。OB-Rb常在很多肿瘤中高表达，包括乳腺癌、胰腺癌和肿瘤起始干细胞（TISCS），在肥胖的肿瘤促进效应中可能具有潜在的机制（2,7）。

原厂资料：

Source / Purification

Recombinant human Leptin (hLeptin) Val22-Cys167 (Accession #NP_000221) was produced in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hLeptin has a Met on the amino terminus and has a calculated MW of 16,026. DTT-reduced and non-reduced protein migrate as 15 kDa polypeptides. The expected amino-terminal MVPIQ of recombinant hLeptin was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLeptin. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hLeptin was determined by its ability to induce TNF-α production from MCF7 cells. The minimum concentration used to obtain this effect was 50 µg/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hLeptin. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Leptin is a 16 kDa adipocyte-derived hormone that relays the status of the body’s energy reserves to the hypothalamus, resulting in the suppression of appetite and modulation of energy expenditure (1,2). Leptin functions as a pleiotropic cytokine with multiple roles in immune function and reproduction (1-4). There are at least five isoforms of the leptin receptor, but only OB-Rb is signaling competent (1,2). OB-Rb is expressed on a number of cells, including T cells, B cells, keratinocytes, and others (1-6). Leptin/OB-Rb interactions induce Jak2, PI3K, Erk1/2, and Stat3 activation (1,2). OB-Rb is often highly expressed in many tumor types, including breast, pancreatic, and Tumor Initiating Stem Cells (TISCS), providing a potential mechanistic link for the tumor promoting effects of obesity (2,7).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.