Phospho-CDC37 (Ser13) (D8P8F) Rabbit mAb

• 产品编号：CST-13248S      品牌：CST       原厂货号：13248S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

</p>Phospho-CDC37 (Ser13) (D8P8F) Rabbit mAb可以识别内源性Ser13被磷酸化的CDC37细胞。</p>单克隆抗体由合成磷酸肽段免疫动物产生，该肽段与人CDC37蛋白Ser13附近氨基酸一致。</p> CDC37是HSP90分子伴侣复合物的一个重要组分（1,2）。最初它被认为参与了细胞周期进程，随后发现它具有更广泛的作用，可以作为多种激酶和蛋白的分子伴侣（1-3）。CDC37蛋白由一个氨基端激酶结合结构域，紧跟其后的是一个中心HSP90结合结构域。CDC37通过保护新合成的激酶肽段使其不被降解从而进入下一步成熟过程，从而募集并稳定了HSP复合物中的激酶（4,5）。CDC37也能抑制HSP90的ATPase活性，因此使复合物发生构象变化而排除靶激酶进入（6）。由于CDC37在增殖和癌细胞生存过程中涉及的多种激酶通路中发挥了重要功能，包括Raf, Akt, Src, 和ErbB2通路，CDC37被作为了治疗靶点（7,8）。</p> CKII磷酸化CDC37的羧基端Ser13，这个磷酸化对于HSP90的结合以及对靶蛋白的稳定功能都是必须的（9,10）。

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22.
2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92.
3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7.
4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28.
5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9.
6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9.
7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85.
8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.
9. Shao, J. et al. (2003) J Biol Chem 278, 38117-20.
10. Miyata, Y. and Nishida, E. (2004) Mol Cell Biol 24, 4065-74.

原厂资料：

Specificity / Sensitivity

<p>Phospho-CDC37 (Ser13) (D8P8F) Rabbit mAb recognizes endogenous levels of CDC37 protein only when phosphorylated at Ser13.

Source / Purification

<p>Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser13 of human CDC37 protein.

Background

<p>CDC37 is an important component of the HSP90 chaperone complex (1,2). It was initially identified for its involvement in cell-cycle progression and was later found to have a much broader role as a chaperone for a wide variety of kinases and other proteins (1-3). CDC37 protein has an amino-terminal kinase binding domain followed by a central HSP90 binding domain. It recruits and stabilizes kinases in the HSP90 complex by protecting the newly synthesized kinase peptide chain from degradation and promoting the next step of protein maturation (4,5). CDC37 also suppresses the ATPase activity of HSP90, thereby leading to conformational changes in the complex that preclude target kinase loading (6). CDC37 has been proposed as a therapeutic target because of its important role in multiple kinase pathways involved in proliferation and cancer cell survival, including Raf, Akt, Src, and ErbB2 pathways (7,8).<p>CDC37 is phosphorylated by CKII at its carboxy-terminal Ser13 residue; this phosphorylation is required for its interaction with HSP90 and target protein stabilization function (9,10).

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22.
2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92.
3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7.
4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28.
5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9.
6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9.
7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85.
8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.
9. Shao, J. et al. (2003) J Biol Chem 278, 38117-20.
10. Miyata, Y. and Nishida, E. (2004) Mol Cell Biol 24, 4065-74.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.