VEGF Receptor 2 (D5B1) Rabbit mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of total VEGF receptor 2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 protein.
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor®555 fluorescent dye and tested in-house for immunofluorescent analysis in rat cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated VEGF Receptor 2 (D5B1) Rabbit mAb #9698.
原厂资料:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 protein.
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor®555 fluorescent dye and tested in-house for immunofluorescent analysis in rat cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated VEGF Receptor 2 (D5B1) Rabbit mAb #9698.
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cellsin vitroand angiogenesisin vivo(6-8).
Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibod-
ies. Protect from light. Do not freeze