Phospho-Src (Ser17) (D7F2Q) Rabbit mAb

• 产品编号：CST-12432S      品牌：CST       原厂货号：12432S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Src (Ser17) (D7F2Q)Rabbit mAb兔单抗能够检测内源性的Ser17磷酸化的Src蛋白。该单克隆抗体是采用合成的与人源Src蛋白Ser17周围的序列相一致的磷酸化肽段免疫动物而产生的。Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的，但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化，能够上调酶活性；而蛋白羧基端尾部Tyr527位点被Csk磷酸化后，则会使酶的活性降低(2)。蛋白激酶A（PKA）依赖的Src的Ser17磷酸化被认为会影响多条信号通路（3-5）。 通过磷酸化蛋白组学的筛选，该位点已被鉴定为mTOR的底物。（6）。

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Schmitt, J.M. and Stork, P.J. (2002) Mol Cell 9, 85-94.
4. Abrahamsen, H. et al. (2003) J Biol Chem 278, 17170-7.
5. Obara, Y. et al. (2004) J Cell Sci 117, 6085-94.
6. Hsu, P.P. et al. (2011) Science 332, 1317-22.

原厂资料：

Specificity / Sensitivity

Phospho-Src (Ser17) (D7F2Q) Rabbit mAb recognizes endogenous levels of Src protein only when phosphorylated at Ser17.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser17 of human Src protein.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).Protein kinase A (PKA)-dependent phosphorylation of Src at Ser17 is thought to influence multiple signaling networks (3-5). This site has also been identified in a phospho-proteomic screen for substrates of mTOR (6).

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Schmitt, J.M. and Stork, P.J. (2002) Mol Cell 9, 85-94.
4. Abrahamsen, H. et al. (2003) J Biol Chem 278, 17170-7.
5. Obara, Y. et al. (2004) J Cell Sci 117, 6085-94.
6. Hsu, P.P. et al. (2011) Science 332, 1317-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody