PathScan? Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit

• 产品编号：CST-12116C      品牌：CST       原厂货号：12116C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

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描述：

PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit 能够检测人的细胞中内源性的磷酸化-ACC (Ser79)水平。本试剂盒可检测经内部测试确定的指定物种的蛋白质，同时也可以检测其他物种的同源蛋白。The PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit采用了固相夹心酶联免疫吸附方法（ELISA）通过化学发光读数检测内源性水平的乙酰辅酶A羧化酶（ACC）蛋白Ser79磷酸化情况。与传统的显色检测法相比，化学发光ELISAs往往有着更宽泛的动态范围和更高的灵敏度。这种化学发光ELISA采用低体积的微孔板，在加强信号和灵敏度的同时需要的样本量更少。微孔中包被了Phospho-ACC (Ser79) Rabbit Antibody。细胞裂解液孵育后，磷酸化ACC蛋白被包被的抗体捕获。充分洗涤后，添加ACC Mouse Detection mAb检测捕获的ACC蛋白。再用Anti-mouse IgG, HRP-linked Antibody识别结合的抗体。添加化学发光试剂增强信号。测得的相对光单位（RLU）与磷酸化ACC（Ser79）蛋白的量成正比。试剂盒中的抗体为该试剂盒所特有。乙酰辅酶A羧化酶（ACC）催化乙酰辅酶A羧化形成丙二酰辅酶A（1）。这是脂肪酸生物合成和氧化过程中的关键酶（1）。在啮齿类动物中，脂肪组织中主要表达的是265 kDa的ACC1（ACCα），而280 kDa的ACC2（ACCβ）是氧化组织中的主要构型（1,2）。然而，在人体中，ACC2是在脂肪和氧化组织均是主要构型（1,2）。AMPK催化的Ser79磷酸化与PKA催化的 Ser1200磷酸化会抑制ACC的酶活性（3）。ACC是抗肥胖药物的潜在靶点（4,5）。

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

原厂资料：

Specificity / Sensitivity

PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit recognizes endogenous levels of phospho-ACC (Ser79) in human cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetyl-CoA carboxylase (ACC) protein phosphorylated at Ser79 with a chemiluminescent readout. Chemiluminescent ELISAs often have a wider dynamic range and higher sensitivity than conventional chromogenic detection. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using smaller samples. A Phospho-ACC (Ser79) Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, phospho-ACC protein is captured by the coated antibody. Following extensive washing, an ACC Mouse Detection mAb is added to detect the captured ACC protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of phospho-ACC (Ser79) protein. 
 Antibodies in kit are custom formulations specific to kit.

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

注意事项：

Low volume microplate * 12 8-well modules -each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).