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pNL3.2.CMV[NlucP/CMV] Vector

 
包装: 20ug
运保温度: –20°C
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其它组分:

pNL3.2.CMV Vector【子货号:N141A,包装:1 x 20μg,,运保温度:–20°C】


描述:

The pNL3.2.CMV Vector(a,b) is designed for constitutive expression of the NanoLuc® reporter fused to a PEST destabilization domain (NlucP). The NlucP fusion protein naturally accumulates at low intracellular levels due to constitutive proteosomal degradation, thus serving as a negative control for experiments configured to measure regulated changes in NanoLuc® luciferase expression levels. This vector also encodes a hygromycin resistance gene for selection in mammalian cells and an ampicillin resistance gene for selection in bacteria. The pNL3.2.CMV Vector contains the following features:
• A CMV promoterfor robust expression in mammalian cells
• The NanoLuc® reporter gene, for high sensitivity detection of the protein of interest.
• PEST sequence for maintaining low intracellular NanoLuc® luciferase concentration.
• Ampicillin resistance gene for selection in bacteria.
• Hygromycin resistance gene for selection in mammalian cells.


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