The CellTiter-Glo® 2.0 Assay provides a homogeneous method for determining the number of viable cells in culture by measuring the amount of ATP present, which indicates the presence of metabolically active cells. The CellTiter-Glo® 2.0 Assay is based on the original CellTiter-Glo® Assay chemistry but with improved storage convenience for easy implementation. The CellTiter-Glo® 2.0 Assay is provided as a single, ready-to-use reagent that can be stored at 4°C for up to 5 months with >90% activity remaining or at room temperature for 1 week with >85% activity remaining. The CellTiter-Glo® 2.0 Assay is designed for use with multiwell plate formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® 2.0 Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent.
The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® 2.0 Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than three hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates.
Features - Benefits
Ready-to-Use Reagent:The single, ready-to-use reagent and convenient storage stability at 4°C or 22°C eliminate reagent thawing and preparation, freeing up resources and time, and allow fast and easy implementation.
Improved Storage Stability:Storage stability at 4°C or room temperature allows the same kit to be used multiple times over several days or weeks while maintaining performance.
Robust:Stable luminescent signal with a half-life >3 hours, depending on cell type and culture medium used, allowing batch processing; delivers excellent Z´-factor values for screening applications.
Flexible:The assay can be used with various multiwell formats (96-well, regular or low-volume 384-well and 1,536-well plates. Reagents are offered in volumes to accommodate low-throughput to high-throughput applications. Data can be recorded by luminometer or CCD camera or other imaging device capable of reading luminescence in multiwell plates.
Able to Multiplex:Can be used with other nonlytic-compatible cell-based assay chemistries from Promega.
Simple Protocol:Uses a simple add-mix-read protocol with just a 10-minute incubation.
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