Magne? Protein A Beads, 20% Slurry

产品编号：G8783 品牌：promega 原厂货号：G8783
产品分类：生化和细胞分析 > 微珠和磁珠 > 配体耦联磁珠 > Protein A&G 磁珠
应用分类：

包装：

50ml

运保温度:

4°C

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其它组分：

Magne™ Protein A Beads, 20% Slurry【子货号：G878B，包装：1 x 50ml，，运保温度：4°C】

描述：

说明：Magne^TMProtein G Beads和Magne^TMProtein A Beads是从细胞培养基，腹水和血清标本纯化免疫球蛋白的高特异性和高容量亲和磁珠。这些顺磁性磁珠由具有低非特异性结合能力的大孔纤维素外壳包裹铁内核组成。该磁珠采用了一种新的附着原理将重组Protein G或Protein A分子以同一方向固定在磁珠表面。定向固定提高了固定蛋白质的功能。这些磁珠提供了从多种生物样品中获得高纯度、高回收率的单克隆抗体和多克隆抗体的简便方法。Magne^TMProtein G Beads和Magne^TM ProteinA Beads拥有极佳的磁力特性，使得无论是手动处理的样品或采用自动化操作平台的高通量方式，如Promega ReliaPrepTM

LV32 HSM仪器或贝克曼Biomek®FX，均能快速、高效地捕获抗体。

特点

• 高容量：根据抗体种类和类型，磁珠结合能力超过25mg/ml。

• 易于处理：最大限度地减少在纯化过程中的损失，磁珠独特的磁

力特性可以增加样品通量。

• 高纯度：非特异性结合极低，以确保高质量的纯化。

• 优化的性能：经验证可纯化从小体积(20µl)到中等体积(50ml)

的样品。

原厂资料：

Magne™ Protein G and Magne™ Protein A Beads are magnetic affinity beads with high specificity and high capacity for purification of immunoglobulins from cell culture media, ascites and serum samples. These paramagnetic beads are composed of iron encapsulated in macroporous cellulose with low nonspecific binding. The magnetic beads use a novel attachment chemistry to immobilize recombinant Protein G or Protein A protein molecules in the same orientation on the surface of the bead. The oriented attachment is known to improve the functionality of immobilized proteins. These beads offer a convenient method for achieving high purity and high recovery of monoclonal and polyclonal antibodies from a variety of biological samples. The superb magnetic properties of Magne™ Protein G and Magne™ Protein A Beads allow rapid and efficient capture of antibodies either with manually processed samples or in a high-throughput manner using the Promega ReliaPrep™ LV 32 HSM Instrument or a robotic platform such as the Beckman Coulter Biomek® FX.

Features - Benefits

High Capacity: Binding capacities in excess of 25mg per milliliter of settled beads are observed depending on antibody species and isotype.
Ease of Handling: Minimize losses during purification and increase sample throughput due to exceptional magnetic properties.
High Purity: Ensure high-quality purification because of low nonspecific binding on beads.
Optimized Performance: Use validated antibody purification methods for small (20µl) to medium (50ml) sample volumes.

Applications

Antibody purification from serum, ascites and cell-media samples.
Automated, high-throughput antibody purification.
Antibody purification and concentration from small-volume or dilute source material.

注意事项：

不要冷冻。在储存或使用过程中不要让磁珠变干。

不推荐将 Magne™ Protein A Beads 用于免疫沉淀（ IP ）或 co-IP 实验。