ADP-Glo? Kinase Assay +LCK Kinase Enzyme System

产品编号：V9491 品牌：promega 原厂货号：V9491
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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其它组分：

LCK Kinase Enzyme System【子货号：V2691，包装：1 x 10μg，，运保温度： –70°C 】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度： –20°C 】

描述：

说明		在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 GST 标签的重组全长人源 LCK 。 LCK （p56lck ）是非受体酪氨酸激酶 src 家族中的一员，编码该蛋白的基因起初被认为在鼠淋巴瘤 LSTRA 中发生重排和过表达，就好像紧挨着这个基因插入莫洛尼鼠白血病病毒 DNA 所导致的结果。像原癌基因一样， Lck 激活后会导致细胞转化。在众多人肿瘤细胞系中，都发现了 LCK 的过表达，表明该激酶可能在人类肿瘤的发生和转移的保持过程中发挥了作用。 ADP-Glo™ Kinase Assay （ ADP-Glo™ 激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。激酶酶系统的组成： • 激酶： LCK , 10μg ( 人源，重组全长蛋白 ) ； MW : ~84kDa 。 • 底物： Poly (4:1 Glu, Tyr) Peptide 。 • 其它组分：反应缓冲液， DTT ， MnCl₂ 。 LCK NCBI 数据库入口。人类激酶酶系统及其应用。
特点		• 在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒与激酶酶系统的优化组合，使您可以迅速方便地开始实验。 • 完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl₂ 。 • 可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。
操作手册		LCK Application Notes. Kinase-Lot-Specific Data Sheets. ADP-Glo™ Kinase Assay Technical Manual #TM313.
储存条件		收到试剂盒后，请将激酶轻微离心后分装成小份，储存于 –70°C ；其他组分储存于 –20°C 。
参考文献		1. Fischer, S. et al. (1987) Biochem. Biophys. Res. Commun. 143(3) , 819–26. 2. Veillette, A. et al. (1987) Oncogene Res. 1(4) , 357–74.
专利/免责声明		For Cat.# V9491: Patents Pending. U.S. Pat. No. 7,770,310, Australian Pat. No. 2003268489 and other patents pending. U.S. Pat. Nos. 6,602,677 and 7,241,584, Australian Pat. Nos. 754312 and 785294 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

原厂资料：

Recombinant full-length human LCK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LCK (p56lck) is a member of the src family of nonreceptor tyrosine kinases. It was identified as a gene rearranged and overexpressed in the murine lymphoma LSTRA, most likely as a result of the insertion of Moloney murine leukemia virus DNA immediately adjacent to the gene. LCK behaves as a proto-oncogene and can lead to cell transformation upon activation. A number of human cancer cell lines show overexpression of LCK, pointing to a possible role for this kinase in the initiation and maintenance of the transformed state in human cancers.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: LCK, 10μg (Human, recombinant full-length). MW: ~84kDa.
Substrate: Poly (4:1 Glu, Tyr) Peptide.
Other: Reaction Buffer, DTT, MnCl2.

LCK NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Fischer, S. et al. (1987) Biochem. Biophys. Res. Commun. 143(3), 819–26.
2.Veillette, A. et al. (1987) Oncogene Res. 1(4), 357–74.