ADP-Glo? Kinase Assay +GSK3β Kinase Enzyme System

产品编号：V9371 品牌：promega 原厂货号：V9371
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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其它组分：

GSK3β Kinase Enzyme System【子货号：V1991，包装：1 x 10μg，，运保温度： –70°C】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度： –20°C】

描述：

说明		在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 His 标签的重组全长人源 GSK3β 。 GSK3β 是一个丝 / 苏氨酸蛋白激酶，最初被认为是一种能磷酸化并抑制糖原合成酶的激酶。 GSK3β 广泛存在于人体组织中，参与多种生理活动的调控，包括胰岛素对糖原和蛋白合成的调控，以及对转录因子 AP-1 和 CREB 的调节。在稳定转染了人源 tau 的单一异构体的黑线仓鼠卵巢细胞中，瞬时转染入 GSK3β 后，可以用磷酸化依赖的 anti-tau 抗体检测到在所有位点的 tau 过磷酸化。 ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。激酶酶系统的组成： • 激酶： GSK3β , 10μg ( 人源，重组全长蛋白 ) ； MW : ~48kDa 。 • 底物： GSK3 底物（ YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE ）；从人肌肉糖原合成酶 1 （第 636 位 – 第 661 位氨基酸）衍生得到。其它组分：反应缓冲液， DTT 。 GSK3β NCBI 数据库入口。人类激酶酶系统及其应用。
特点		• 在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒与激酶酶系统的优化组合，使您可以迅速方便地开始实验。 . 完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl₂。 • 可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。
操作手册		GSK3β Application Notes. Kinase-Lot-Specific Data Sheets. ADP-Glo™ Kinase Assay Technical Manual #TM313.
储存条件		收到试剂盒后，请将激酶轻微离心后分装成小份，储存于 –70°C ；其他组分储存于 –20°C 。
参考文献		1. Sutherland C. et al. (1993) Biochem. J. 296 (Pt 1) , 15–9. 2. Sperber B.R. et al. (1995) Neurosci. Lett. 197(2) , 149–53.
专利/免责声明		For Cat.# V9371: Patents Pending. U.S. Pat. No. 7,770,310, Australian Pat. No. 2003268489 and other patents pending. U.S. Pat. Nos. 6,602,677 and 7,241,584, Australian Pat. Nos. 754312 and 785294 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

原厂资料：

Recombinant full-length human GSK3β was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3β is a serine-threonine protein kinase that was originally identified as the kinase that phosphorylates and inhibits glycogen synthase. GSK3β is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin, modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3β into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: GSK3β, 10μg (Human, recombinant full-length). MW: ~73kDa.
Substrate: GSK3 Substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE); derived from human muscle glycogen synthase 1 (amino acid 636–661).
Other: Reaction Buffer, DTT.

GSK3β NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Sutherland C. et al. (1993) Biochem. J. 296 (Pt 1), 15–9.
2.Sperber B.R. et al. (1995) Neurosci. Lett. 197(2), 149–53.