Anti-ACTIVE? JNK pAb, Rabbit, (pTPpY)

产品编号：V7931 品牌：promega 原厂货号：V7931
产品分类：抗体 > 一抗 > 磷酸化抗体
应用分类：

包装：

40μl

运保温度:

–20°C

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其它组分：

Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY)【子货号：V793A，包装：1 x 40μl，，运保温度：–20°C】

描述：

说明

Anti-ACTIVE^` JNK pAb 是来源于兔血清的多克隆抗体。该抗体通过相当于JNK 酶活性的双磷酸化肽亲和纯化。

特点

• 特异性：特异性检测双重磷酸化的、应激激活的蛋白激酶(stress-activated protein kinase， SAPK) 的活性形式，即c-Jun N-terminal kinase,JNK。

• 免疫原：Thr/Pro/Tyr 区域(pTPpY) 双重磷酸化来源于JNK 激酶活性形式的催化中心，相当于哺乳动物JNK2 酶的Thr¹⁸³和Tyr¹⁸⁵。

• 抗体形态：亲和纯化的兔IgG；溶于10mM Na₃PO₄(pH 7.4) 及20mM NaCl。

• 物有所值：Anti-ACTIVE^® JNK pAb 有2 种方便的包装。产品Cat.# V7931 可制备200ml blotting 溶液，每次使用10ml，足够完成20 次Western blot 实验。大包装产品Cat.# V7932 可制备600ml blotting 溶液，每次使用10ml，足够完成60 次Westernblot 实验。

原厂资料：

Anti-ACTIVE® JNK pAb is a polyclonal antibody from rabbit serum. The antibody is affinity purified using a dually phosphorylated peptide that corresponds to the active form of the JNK enzymes.

Features - Benefits

Specificity: Preferentially detects the dually phosphorylated, active form of the stress-activated protein kinase (SAPK), also known as c-Jun N-terminal kinase, JNK.
Immunogen: Dually phosphorylated Thr/Pro/Tyr region (pTPpY) derived from the catalytic core of the active form of JNK kinase, which corresponds to Thr183 and Tyr185 of the mammalian JNK2 enzyme.
Antibody Form: Affinity-purified rabbit IgG; supplied in 10mM sodium phosphate (pH 7.4), 20mM NaCl.
Value: Anti-ACTIVE® JNK pAb is available in two convenient sizes. Cat.# V7931 will generate up to 200ml of blotting solution, sufficient for 20 Western blots of 10ml each. The larger size, Cat.# V7932, will generate up to 600ml of blotting solution, sufficient for 60 Western blots of 10ml each.

Applications

(Suggested working dilution)
Western blotting (1:5,000).
Immunocytochemistry (1:1,000).
Immunohistochemistry (1:500).