Western Blue® 稳定型碱性磷酸酶底物是一种稳定、即用的底物，用于Western blots 及免疫筛选。Western Blue® 底物应直接使用而不需稀释。它是5- 溴-4- 氯-3- 吲哚- 磷酸(BCIP) 与四唑硝基蓝(NBT) 在专门的稳定缓冲液中的混合物。该液体底物在碱性磷酸酶的膜位点上会留下永久的深紫色染色。Western Blue® 底物与BCIP/NBT 试剂同样灵敏。
特点
• 方便：可以直接使用而无须稀释或混合。
• 灵敏度：底物与其它的BCIP/NBT 试剂同样灵敏度。
• 稳定：在室温下可以稳定存放一年。
原厂资料：
Western Blue® Stabilized Substrate for Alkaline Phosphatase is a stable, ready-to-use substrate for Western blots and immunoscreening. It is a mixture of 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitro blue tetrazolium (NBT) in a proprietary stabilizing buffer. Western Blue® Substrate should be used directly and without dilution. This liquid substrate deposits a permanent dark purple stain on membrane sites bearing alkaline phosphatase. Western Blue® Substrate is as sensitive as other reagents based on the BCIP/NBT formulation.
Features - Benefits
Convenient: Ready-to-use formulation that does not require dilution or reagent mixing.
Sensitive: Substrate is as sensitive as other commercially available BCIP/NBT formulations and reagents.
Stable: Stable for one year at room temperature.
Applications
Western blotting.
Dot blotting.
References
Cedeno-Laurent, F. et al. (2012) Galectin-1 triggers an immunoregulatory signature in Th cells functionally defined by IL-10 expression. J. Immunol. 188, 3127–37.
Petrova, N.S. et al. (2012) Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group. Nucleic Acids Res. 40, 2330–44.
To, W.S. and Midwood, K.S. (2011) Identification of novel and distinct binding sites within Tenascin-C for soluble and fibrillar fibronectin. J. Biol. Chem. 286, 14881–91.
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