Coelenterazine-h

产品编号：S2011 品牌：promega 原厂货号：S2011
产品分类：生化和细胞分析 > 标签、标记试剂盒 > 酶底物 > 荧光素酶底物
应用分类：

包装：

250μg

运保温度:

–20°C

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其它组分：

Coelenterazine-h【子货号：S201A，包装：1 x 250μg，，运保温度：–20°C】

描述：

说明

来自海肾(Renilla )、Aequorea 和其它海洋生物的萤光素酶，通常用作真核细胞表达分析的细胞现象的指示剂或报告蛋白。海肾萤光素酶经常被用作转录调控的报告子，而apoaequorin 经常被用作钙指示剂。Coelenterazine ( 腔肠素) 的其它应用还包括在细胞或组织中用化学发光法检测Reactive Oxygen Species (ROS)。Promega还提供多种腔肠素类似物，包括：

Coelenterazine(native)：海肾萤光素酶和apoaequorin 的发光底物。分子式：C₂₆H₂₁N₃O₃。分子量：423.5。形态：冻干薄膜状。

Coelenterazine-h：是一种水母发光蛋白复合物，其发光强度比野生型腔肠素高10-20 倍，是检测Ca2+ 浓度细微变化的有力工具。分子式：C₂₆H₂₁N₃O₂。分子量：407.5。形态：冻干薄膜状。

特点

• 高纯度：≥ 95%。

• 可定制：可按客户要求包装。

• 易制备：提供的是干燥底物，易于在甲醇或乙醇中制备。

• 可定制或批量订购：想了解更多此产品的定制信息，请浏览:www.promega.com/myway/。

原厂资料：

Luciferases from Renilla, Aequorea and other marine organisms are commonly used as indicators or reporters for studying cellular phenomena in expression assays in eukaryotic cells. Renilla luciferase is often used as a reporter of transcription regulation, whereas apoaequorin is often used as a calcium indicator. Other uses of coelenterazines include chemiluminescent detection of Reactive Oxygen Species (ROS) in cells or tissues. Promega offers the following coelenterazine analogs.

Coelenterazine (native) is the luminescent substrate for Renilla luciferase and apoaequorin. Formula: C₂₆H₂₁N₃O₃. Formula Weight: 423.5. Form: Film.

Coelenterazine-h imparts a luminescent intensity with its aequorin complex that is reported to be 10–20 times higher than that of native coelenterazine, making this derivative a useful tool for measuring small changes in Ca²⁺ concentrations. Formula: C₂₆H₂₁N₃O₂. Formula Weight: 407.5. Form: Film.

Features - Benefits

Highly Pure: 95%.
Custom Capabilities: Custom packaging and sizes available.
Easy to Prepare: Supplied as a dried substrate for easy preparation in methanol or ethanol.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

References

Inouye, S. et al. (1997) Biochem. and Biophys. Res. Comm. 233, 349–53.
Blanpain, C. et al. (2001) J. Biol. Chem. 276, 23795–804.
Shimomura, O. et al. (1989) Biochem. J. 261, 913–20.