TNT? T7 Insect Cell Extract Protein Expression System

• 产品编号：L1101      品牌：promega       原厂货号：L1101
• 产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 无细胞表达系统
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The TnT® T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for the cell-free expression of proteins. Protein synthesis reactions are initiated by the addition of a DNA template, eliminating the need for the time-consuming process of in vitro RNA synthesis.

The extract is made from the commonly used Spodoptera frugiperda Sf21 cell line. All components necessary for the transcription/translation are present in the TnT® T7 ICE Master Mix. To initiate protein synthesis, the only component that must be added is the DNA template. Reactions are incubated at 28–30°C and are complete within 4 hours.

Proteins are expressed from genes cloned downstream of the T7 promoter. Companion vectors have been designed to achieve optimal yield with this system (pF25A and pF25K). They contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency. Using the TnT® T7 Insect Cell Extract Protein Expression System and these vectors, 75μg/ml of functional protein can be produced.

Features - Benefits

• Obtain Data Faster: Protein is expressed in only 4 hours, not days as with cell-based expression.
• Complete System: No requirement to purchase additional reagents.
• Achieve High Protein Yields: Express up to 75μg/ml of protein for multiple applications.

Applications

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.