CellTiter-Glo? One Solution Assay

• 产品编号：G8461      品牌：promega       原厂货号：G8461
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞活性及活力检测试剂盒
• 应用分类：

描述：

原厂资料：

The CellTiter-Glo® One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells. This frozen, ready-to-use format is based on the original CellTiter-Glo® Luminescence Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. The CellTiter-Glo® Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) in 96- to 1536-well format, and cell proliferation and cytotoxicity assays.

The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® One Solution Assay generates a stable "glow-type" luminescent signal with a half-life of greater than three hours. This extended half-life eliminates the need for reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates.

Features - Benefits

Convenient: No reagent preparation is required; simply thaw and "add-mix-measure". Volumes convenient for HTS applications.
Homogeneous: "Add-mix-measure" format reduces the number of plate-handling steps.
Fast: Data can be recorded 10 minutes after reagent addition.
Sensitive: Measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays.
Flexible: Can be used with various multiwell formats (96-, regular or low-volume 384- and 1536-well plates). Data can be recorded by luminometer or CCD camera imaging device.
Robust: Stable luminescent signal with a half-life >3 hours, depending on cell type and culture medium used.
Ability to Multiplex: Can be used with other nonlytic compatible assay chemistries from Promega.

Applications

Cell viability.
Cytotoxicity.
Cell proliferation.
High-throughput screening.

Notes

Cat.# G8461 is sufficient for 1,000 assays at 100µl/assay in 96-well plates or 4,000 assays at 25µl/assay in 384-well plates.
Cat.# G8462 is sufficient for 5,000 assays at 100µl/assay in 96-well plates or 20,000 assays at 25µl/assay in 384-well plates.

References

Farfan, A. et al. (2004) Multiplexing homogeneous cell-based assays. Cell Notes 10, 2–5.
Riss, T., Moravec, R. and Niles, A. (2005) Selecting cell-based assays for drug discovery screening. Cell Notes 13, 16-21.

注意事项：

CellTiter-Glo ® One Solution Assay 储存于 –10°C 以下。 CellTiter-Glo ® One Solution Assay 可以在 4°C 存放 48 小时或在 22°C 存放 10 小时，约损失 10–12% 的活性。在第一次解冻后 CellTiter-Glo® One Solution Assay 还可以进行两次冻融，每次冻融会损失约 10% 的活性。

Cat.# G8461 足够在 96 孔板中进行 1,000 次 100µl 体系的实验或在 384 孔板中进行 4,000 次 25µl 体系的实验。