TMB One Solution(TMB 单溶液) 是一种显色底物，3,3`, 5,5`-四甲基联苯胺(TMB) 溶于弱酸性无毒缓冲液中，可用于ELISA 实验中辣根过氧化物酶的检测。该底物被过氧化物酶氧化后形成蓝色反应产物，并且在实验结束时，加入酸性溶液后，在终点多孔检测中形成黄色反应产物。
特点
•方便：即用型的单一溶液，只需加入、孵育、终止及读取结果。单一的试剂可减少不同重复测量之间的变异。
•稳定：4℃下可稳定保持12 个月，其有效期更长；实验最终产物可在终止实验后最少1 小时保持稳定。
•安全：溶于弱酸性无毒的专利缓冲液中，无质子惰性溶剂；对塑料无腐蚀作用，可用于自动化操作系统。
•灵敏：低背景提高实验灵敏度。
原厂资料：
TMB One Solution is a chromagen substrate, 3,3´,5,5´-tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a single solution at a ready-to-use working dilution. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user.
Features - Benefits
Convenient:Single solution provided ready-to-use; just add, incubate, stop and read. This homogeneous reagent improves assay variation.
Stable:Stable for 12 months at 4°C, providing extended shelf life; the assay end product is stable for at least one hour after stopping the assay.
Safe:Provided in a slightly acidic, nonhazardous proprietary buffer without aprotic solvents; noncaustic to plastics used in automated systems.
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