The Caspase-Glo® 6 Assay is a homogeneous, luminescent assay that measures caspase-6 activity. Caspase-6 is a member of the cysteine aspartic acid-specific protease family and has a key effector role in the cleavage of specific target proteins during apoptosis. The Caspase-Glo® 6 Assay provides a luminogenic substrate, Z-VEID-aminoluciferin, in a buffer optimized for caspase-6 and luciferase activity. The addition of a single Caspase-Glo® 6 Reagent in an add-mix-measure format results in cleavage of the substrate, releasing aminoluciferin, and generation of a glow-type luminescent signal in the presence of Ultra-Glo™ Recombinant Luciferase. The luminescent signal is proportional to the amount of caspase-6 activity present. The homogeneous Caspase-Glo® 6 Assay is designed for use with purified enzyme preparations in multiwell plate formats, making it ideal for automated high-throughput screening for caspase-6 activity and inhibitors of caspase-6 activity.
Features - Benefits
Simplified Method: The homogeneous "add-mix-measure" protocol makes the assay highly amenable to automation.
Greater Sensitivity: The assay is more sensitive than fluorescence-based caspase-6 assays. This bioluminescent assay avoids inherent fluorescent background signals, providing excellent signal-to-noise ratios. The assay is linear over 3 logs of caspase-6 concentration and can detect 0.002U/ml.
Increased Accuracy: The superior sensitivity over fluorescence-based caspase assays allows inhibitor studies at concentrations below the Km.
Faster Results: The maximum signal (and maximum sensitivity) of the assay is reached in as little as 30 minutes after reagent addition.
High-Quality Assay: The assay demonstrates an excellent Z´-factor value of 0.86 when using 0.1U/ml of caspase-6 for assays in 384-well plates.
Batch Processing Capability: The coupled-enzyme, homogeneous format results in a continuous signal, providing excellent stability and allowing plates to be read over an extended period of time.
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