GoTaq? Probe 1-Step RT-qPCR System

• 产品编号：A6120      品牌：promega       原厂货号：A6120
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > qRT-PCR
• 应用分类：实时定量 PCR > 一步法RT-qPCR

其它组分：

描述：

原厂资料：

The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix included in the GoTaq® Probe 1-Step RT-qPCR System is formulated with dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); this allows users to incorporate UNG into subsequent reactions for control of possible carryover contamination.

The GoTaq® Probe qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add reference dye to amplification reactions if desired.

The GoTaq® Probe qPCR Master Mix provides resistance to a wide range of PCR inhibitors. This formulation uses antibody-mediated hot-start chemistry, allowing reaction setup to be performed at room temperature. The master mix also employs rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs.

Features - Benefits

• Superior Performance: Sensitive detection on any real-time instrument.
• Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection.
• Versatility: Compatible with both fast and standard cycling methods.
• Confidence: The Promega PCR Performance Guarantee.

Guarantee

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests preformed.

Applications

• Sensitive sequence detection and quantification.
• Gene expression analysis.
• Detection of sequence variants.
• Fluorescent detection.

注意事项：

将所有组分于 -30 ℃至 -10 ℃避光 保存 。为获得最佳结果，请将融化的预混液轻轻混匀，以减少气泡，并于冰上放置。如经常使用或仅短期储存， 2 X GoTaq® qPCR Master Mix 可在 2–8°C 避光保存 3 个月。