ADP-Glo? Kinase Assay + MET(M1250T) Kinase Enzyme System

产品编号：V4169 品牌：promega 原厂货号：V4169
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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1 each

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其它组分：

MET (M1250T) Kinase Enzyme System【子货号：V4168，包装：1 x 10μg，，运保温度：-70℃】

ADP-Glo™ Kinase Assay【子货号：V9101，包装：1 x 1,000 assays，，运保温度：-20℃】

描述：

Recombinant human MET (point mutant M1250T; amino acids 956–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MET is a proto-oncogene that encodes a transmembrane growth factor receptor, which is a heterodimer of two disulphide-linked chains of 50kDa (α) and 145kDa (β). MET is widely expressed in the kidney, brain, lung, skin and embryonic tissue. Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian and has been shown to be involved in tumor cell migration and invasion.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: MET (M1250T), 10μg (Human, recombinant; amino acids 956–end). MW: ~81kDa.
Substrate: Poly (4:1 Glu, Tyr) peptide.
Other: Reaction Buffer, DTT, MnCl2.

MET (M1250T) NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1.Giordano, S. et al. (1989) Biosynthesis of the protein encoded by the c-met proto-oncogene. Oncogene 4, 1383–8.
2.Iyer, A. et al. (1990) Structure, tissue-specific expression, and transforming activity of the mouse met protooncogene. Cell Growth Differ. 1, 87–95.