ADP-Glo? Kinase Assay +CK1γ1 Kinase Enzyme System

• 产品编号：V4101      品牌：promega       原厂货号：V4101
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
• 应用分类：

其它组分：

描述：

Recombinant human CK1 gamma 1 (21–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1γ1 or casein kinase 1, gamma 1 is one of the most abundant serine/threonine kinase in eukaryotic cells and is mainly involved in growth and morphogenesis. CK1γ1 possesses the C-terminal sequence motif (MTM), which it shares with CSNK1G2 and CSNK1G3; this motif is associated with heterologous carboxy-terminal sequences (1). CK1γ1 couples Wnt receptor activation to the cytoplasmic signal transduction apparatus (2).
 

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
 

Kinase Enzyme System contains:
 

Kinase: CK1γ1, 10μg (Human, recombinant; amino acids 21–end). MW: ~70–76kDa.
Substrate: Native Casein Protein; purified from bovine milk.
Other: Reaction Buffer, DTT.
 

CK1γ1 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
 

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
 

References

1.Kusuda, J. et al. (2000) Cloning, expression analysis and chromosome mapping of human casein kinase 1 gamma-1 (CSNK1G1): identification of two types of cDNA encoding the kinase protein associated with heterologous carboxy-terminal sequences. Cytogenet. Cell Genet. 90, 298–302.
2.Davidson, G. et al. (2005) Casein kinase 1-gamma couples Wnt receptor activation to cytoplasmic signal transduction. Nature 438, 867–72.

原厂资料：