AccessQuick? RT-PCR System

• 产品编号：A1703      品牌：promega       原厂货号：A1703
• 产品分类：克隆 > cDNA合成，cDNA文库和文库构建 > cDNA合成
• 应用分类：PCR > RT-PCR > 一步法 RT-PCR

其它组分：

描述：

AccessQuick™ RT-PCR System(AccessQuick™RT-PCR 系统) 是一种简单、方便的RT-PCR 反应系统。RT-PCR 反应在一个试管中就能进行。为使RT-PCR更方便，把Tfl DNA 聚合酶、dNTPs、MgSO4 及反应缓冲液这些组分放在一个管中。AMV 逆转录酶单独放置在另一个管中，以便进行无逆转录的对照反应。采用该系统进行RT-PCR 反应时，只需将AccessQuick™ Master Mix 加入到含有RNA 模板的反应管中，而后加入AMV 逆转录酶和引物即可。AccessQuick™ 反应混合物经过优化，可用于常规RT-PCR 反应，不需其它特别条件。

特点
 
最方便：能减少四步移液操作步骤。只需将AccessQuick™ Master Mix、AMV RT、您的特异引物、RNA 模板以及水简单地混合。AMV RT另外包装,可进行不含RT 的对照反应。

使用更少的模板：能以zeptomole (10-21mol) 水平的RNA 为模板扩增。

不需要增加缓冲液：在一个试管中建立反应，放进热循环仪，稍后再来取结果——在逆转录和DNA扩增之间不需要加缓冲液。

稳定:多次冻融后系统组分仍旧保持稳定。

原厂资料：

The AccessQuick™ RT-PCR System is an easy and convenient master mix system for setting up one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. The AccessQuick™ Master Mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.

Features - Benefits

• Maximum Convenience: Save yourself four pipetting steps. Simply combine the AccessQuick™ Master Mix, AMV RT, your gene-specific primers, your RNA template and water. Separate AMV RT allows important no-RT control reactions.
• Less Template: Amplify from zeptomole (10^–21mol) levels of RNA.
• No Buffer Additions Required: Set up reactions in a single tube, place in the thermal cycler and come back later for results—no additions between the reverse transcription and DNA amplification steps.
• Stability: System components are stable over many freeze-thaw cycles.

Guarantee

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests preformed.

Applications

For more information, see the Protocols & Applications Guide.

注意事项：