PureYield? Plasmid Miniprep System

产品编号：A1223 品牌：promega 原厂货号：A1223
产品分类：DNA和RNA纯化 > DNA纯化 > 质粒DNA纯化
应用分类：核酸分离纯化与分析 > DNA纯化 > 质粒 DNA 纯化
　　　　　细胞培养产品与服务 > 转染 > 转染试剂的选择 > 样品类型 > 质粒DNA（环化DNA) 转染

包装：

100prep

运保温度:

22–25°C

到货周期：

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其它组分：

Cell Lysis Buffer (CLC)【子货号：A145D，包装：1 x 27ml，，运保温度：22–25°C】

Neutralization Solution (NSC)【子货号：A157D，包装：1 x 90ml，，运保温度：22–25°C】

Endotoxin Removal Wash (ERB)【子货号：A158D，包装：1 x 20.5ml，，运保温度：22–25°C】

Column Wash Solution (CWC)【子货号：A159D，包装：1 x 9ml，，运保温度：22–25°C】

Elution Buffer (EBB)【子货号：A160D，包装：1 x 10ml，，运保温度：22–25°C】

PureYield™ Minicolumns【子货号：A166B，包装：2 x 50 columns，，运保温度：22–25°C】

PureYield™ Collection Tubes【子货号：A167B，包装：2 x 50 tubes，，运保温度：22–25°C】

描述：

说明

PureYield^™ Plasmid Miniprep System (PureYield^™质粒小提系统)能快速抽提高纯度质粒DNA。从600μl到3ml细菌培养物中仅需10分钟就能纯化出高达15μg的质粒DNA。PureYield^™质粒小提系统带有独特的内毒素去除洗涤液，能从纯化的质粒DNA中去除大量的蛋白、RNA和内毒素污染物。污染物的去除使某些对质粒纯度要求高的下游实验变得更加强劲，这些应用诸如真核细胞转染、体外转录和偶联的转录/翻译(例如，
TNT^® Quick Coupled Transcription/Translation System)。纯化的质粒无需异丙醇沉淀或长时间的离心步骤，提供了一种快速简单的纯化方法。

本系统可使用离心或真空法(例如，Vac-Man^® Laboratory Vacuum Manifold)。

特点

. 提高生产力：快速实验方案纯化质粒DNA 仅需10 分钟。

• 强劲的性能：高纯度和高浓度的质粒DNA 在转染、无细胞表达以及其它分子生物学应用上性能可靠。

• 结果可靠：裂解液/中和液指示染料确保了每次实验都成功。

• 灵活：既可选择使用离心方法，又可选择抽真空法。

原厂资料：

The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15μg of plasmid DNA from 600μl to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield™ Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TnT® Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.
The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold).

This system can be used to isolate any plasmid from E. coli hosts but works most
efficiently when the plasmid is up to 20,000bp in size. For best results, use
plasmids that are 10,000bp or less. Purified plasmid can be used without further
manipulation for automated fluorescent DNA sequencing as well as for other
standard molecular biology techniques. When used for in vitro transcription
reactions, the isolated plasmid DNA should be supplemented with a
ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor
(Cat.# N2511). The protocol presented in this Technical Bulletin describes
isolation of plasmid DNA from E. coli. Plasmid yield will vary, depending on a
number of factors, including culture volume, plasmid copy number, type of
culture medium and bacterial strain used.

PureYield™ Plasmid Miniprep System 250 preps A1222
Includes:
• 27ml Cell Lysis Buffer (CLC) (Blue)
• 90ml Neutralization Solution (NSC)
• 2 × 27ml Endotoxin Removal Wash (ERB)
• 25ml Column Wash Solution (CWC)
• 10ml Elution Buffer (EBB)
• 250 PureYield™ Minicolumns
• 250 PureYield™ Collection Tubes

注意事项：

储存条件

将所有系统组分储存于22-25℃。

Note: Throughout the remainder of this document, the supplied Cell Lysis
Buffer (CLC), Neutralization Solution (NSC), Endotoxin Removal Wash (ERB),
Column Wash Solution (CWC) and Elution Buffer (EBB) are referred to as Cell
Lysis Buffer, Neutralization Solution, Endotoxin Removal Wash, Column Wash
Solution and Elution Buffer, respectively.