Senescence β-Galactosidase Staining Kit

• 产品编号：CST-9860S      品牌：CST       原厂货号：9860S
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞增殖及周期检测试剂盒
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描述：

.该试剂盒检测培养细胞中pH为6时β-galactosidase 的活性。pH 6时β-galactosidase 的活性仅见于衰老细胞，未见于衰老前细胞、静止细胞或永生化细胞。.Senescence β-Galactosidase Staining Kit设计为pH 6时检测β-galactosidase 活性，此活性为衰老细胞独有，未见于非衰老细胞、静止细胞或永生化细胞。该试剂盒包括该检测所需所有试剂。复制能力的有限性是大多数正常细胞一个典型特征，最终以衰老结束，一种细胞仍然存活的阻滞状态(1)。衰老细胞不接受培养过程中血清或传代的刺激而分化，而且衰老能够引起特定的细胞周期属性，不同于大多数损伤诱导的阻滞或接触抑制(2)。增大的细胞体积，pH-依赖的β-半乳糖苷酶活性表达(3)，基因表达模式的改变(4,5)进一步表征了衰老细胞。

1. Goldstein, S. (1990) Science 249, 1129-1133.
2. Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.
3. Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.
4. Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
5. Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.

原厂资料：

Specificity / Sensitivity

The kit detects β-galactosidase activity at pH 6 in cultured cells. β-galactosidase activity at pH 6 is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.

Description

The Senescence β-Galactosidase Staining Kit is designed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). The kit includes all reagents necessary for this assay.

Background

Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells. 

1. Goldstein, S. (1990) Science 249, 1129-1133.
2. Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.
3. Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.
4. Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
5. Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.