Apoptosis Marker: Cleaved Caspase-3 (Asp175) Western Detection Kit

• 产品编号：CST-9660S      品牌：CST       原厂货号：9660S
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞凋亡试剂盒
• 应用分类：

其它组分：

描述：

Cleaved Caspase-3 (Asp175) Antibody 能够检测活化的邻近Asp175处裂解的caspase-3大片段(17/19 kDa)。该抗体不能识别全长的caspase-3和其它caspases裂解产物。Caspase-3 Antibody 能够检测内源的全长caspase-3 (35 kDa)和裂解后大片段(17 kDa)。该多克隆抗体是用合成的人源caspase-3裂解位点氨基酸序列和大片段氨基末端序列相应的肽段免疫动物后制备获得。抗体通过蛋白A和肽段亲和层析而纯化。凋亡标志物：Cleaved Caspase-3 (Asp175) Western 检测试剂盒，是通过Western blotting检测caspase-3加工和活性的有效方法。该试剂盒含有足够的一抗和二抗的量，可以完成10次Western mini blots，同时还附带有一套生物素标记的预染marker、细胞裂解液和LumiGLO® 试剂。Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者，因为它对许多关键的蛋白起着部分或全部的裂解作用，比如核酶多聚(ADP-ribose)聚合酶(PARP)（1）。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点（2）。

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

原厂资料：

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to (Asp175). This antibody does not recognize full length caspase-3 or other cleaved caspases. Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site and the amino terminus of the large fragment of human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.

Description

Apoptosis Marker: Cleaved Caspase-3 (Asp175) Western Detection Kit offers an efficient way of detecting caspase-3 processing and activation by Western blotting. The kit contains enough primary and secondary antibodies to perform 10 Western mini blots, as well as a set of pre-stained and biotinylated markers, cell lysates and LumiGLO® reagent.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody