Human Interleukin-22 (hIL-22)

• 产品编号：CST-8931SF      品牌：CST       原厂货号：8931SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司生产的人源重组蛋白IL-22 (hIL-22) Ala34-Ile179 (Accession #NP_065386)是从人源293细胞表达而来。重组的hIL-22蛋白在末端没有标签，未糖基化蛋白的分子量据推算为16,749Da。DTT-降解蛋白和未降解的蛋白作为一个34kDa的多肽而转移。其低迁移率和在SDS胶中的异质性是因为糖基化的作用。重组hIL-22蛋白的N-末端APISS的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hIL-22通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白IL-22的生物活性是通过诱导COLO 205细胞产生IL-10 的能力实验来确定的。每个批次的ED50值在100-300 pg/ml之间。

有载体: 每微克hIL-22蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-22蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-22, 是IL-10家族的一员 (1,2),Th17 CD4+ T 细胞、激活的T细胞、Th1 细胞和NK 细胞都能表达IL-22(3)。IL-22诱导促炎症反应，促使抗细菌多肽的产生，同时也参与到组织修复和伤口愈合过程中(1)。IL-22的受体是 IL-22R1和IL-10R2异源二聚体(4)。IL-22R的表达局限于组织细胞中尤其是上皮起源的细胞中, 然而IL-10R2链在更多的细胞类型中表达。IL-22 诱导Jak1和 Tyk2的磷酸化,激活Stat3 和小范围的Stat1和Stat5 (1)。IL-22 反应参与到MEK-ERK-RSK, JNK-SAPK和 p38 信号通路的激活过程中(1)。IL-22表达的升高与Crohn's 疾病和类风湿性关节炎有关系。IL-22 在宿主抵抗肺部细菌克雷伯菌的响应中发挥了重要的作用(5)。

原厂资料：

Source / Purification

Recombinant human IL-22 (hIL-22) Ala34-Ile179 (Accession #NP_065386) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-22 contains no "tags" and the nonglycosylated protein has a calculated MW of 16,749. DTT-reduced and non-reduced protein migrate as 34 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal APISS of recombinant hIL-22 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-22. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant IL-22 was determined by its ability to induce IL-10 production by COLO 205 cells. The ED50 of each lot is between 100-300 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg hIL-22.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-22. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (4). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.