Human Stromal Cell-derived Factor 1β/CXCL12 (hSDF1β)

• 产品编号：CST-5712LC      品牌：CST       原厂货号：5712LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它细胞因子和生长因子
• 应用分类：

描述：

CST公司在大肠杆菌中生产重组人源蛋白SDF1β (hSDF1β) Lys22-Met93 (Accession #NP_000600)。重组的hSDF1β蛋白在N-末端有Met标签，其分子量据推算为8,657Da。DTT-还原蛋白迁移时是作为一个8 kDa 的多肽而转移，未还原的蛋白作为12kDa多肽而转移。重组hSDF1β蛋白的N-末端MKPVS序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hSDF1β通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hSDF1β蛋白的生物活性是同过NK-92 细胞的迁移实验确定的。每个批次的ED50在0.1-0.3 µg/ml之间。内毒素含量:<0.01 ng /1 μg hSDF1β。

有载体：每微克hSDF1β蛋白溶解在包含 100 mM NaCl 和 20 μg BSA的20 mM 柠檬酸盐（pH 3.0）溶液中 ，并通过 0.22 μm 滤膜冻干。

无载体：每微克hSDF1β蛋白溶解在20 mM 柠檬酸盐（pH 3.0）溶液中 ，并通过 0.22 μm 滤膜冻干。

SDF1又叫CXCL12和PBSF，是一个8 kDa的CXC家族成员细胞因子(1)。SDF1在不同的物种中高度保守，在人和小鼠中只有一个保守的氨基酸替代(2)。SDF1α和SDF1β 时间是不同的剪切形式，其氨基酸序列是相同的，只是SDF1β在C-末端多四个氨基酸(3)。两种SDF1形式都能与G蛋白偶联受体CXCR4结合(4)。SDF1β是一个有效的淋巴细胞化学诱导剂(5)。SDF1/CXCR4信号通路是B淋巴细胞增殖和骨髓组织形成所必须的(6,7)。CXCR4信号在乳腺癌转移中发挥了重要的作用，SDF1在乳腺癌中表达的升高可能是生存率的一个指示值(8)。

原厂资料：

Source / Purification

Recombinant human SDF1β (hSDF1β) Lys22-Met93 (Accession #NP_000600) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hSDF1β has a Met on the amino terminus and has a calculated MW of 8,657. DTT-reduced protein migrates as an 8 kDa polypeptide and non-reduced protein migrates as a 12 kDa polypeptide. The expected amino-terminal MKPVS of recombinant hSDF1β was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hSDF1β. All lots are greater than 98% pure.

Bioactivity

The activity of hSDF1β was determined using an NK-92 cell migration assay. The ED50 of each lot is between 0.1-0.3 µg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hSDF1β.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hSDF1β. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

SDF1, also known as CXCL12 and PBSF, is an 8 kDa member of the CXC family of chemokines (1). SDF1 is highly conserved across species and the human protein differs from mouse by only one conserved amino acid substitution (2). SDF1α and SDF1β are splice variants which are identical with regard to amino acid sequence except for an additional 4 amino acids on the carboxy terminus of SDF1β (3). Both SDF1 isoforms binds to the G protein-coupled receptor, CXCR4 (4). SDF1β is a potent lymphocyte chemoattractant (5). SDF1/CXCR4 signaling is required for B cell lymphopoiesis and myelopoiesis (6,7). While CXCR4 signaling plays a key role in breast cancer metastasis, increased SDF1 expression in breast cancer may be a prognostic indicator of overall survival (8).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at ‑20ºC for 6 months. Avoid
repeated freeze‑thaw cycles.