Phospho-TBK1/NAK (Ser172) (D52C2) XP? Rabbit mAb

• 产品编号：CST-5483S      品牌：CST       原厂货号：5483S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb 只能检测内源的在Ser172位点磷酸化的TBK1蛋白。此单克隆抗体是通过合成人源对应的TBK1 Ser172位点周围的肽段来免疫动物而获得。TBK1 (TANK-结合激酶1)/NAK (NF-κB 激活激酶)是IκB激酶(IKK)的激活激酶，能够通过直接磷酸化激活IKK(1)。 TBK1的鉴定是通过与之相关的TRAF结合蛋白——TANK, 并发现在NF-κB的激活中在NIK和IKK 的上游起作用(2)。TBK1/NAK 诱导IκB 的降解和通过IKKβ而激活 NF-κB。生长激素刺激PKCε的活性，NAK 可能是对此作出应答并介导IKK 和 激活NF-κB(1)。TBK1在天然免疫激活IRF3的过程中发挥了重要的作用(3)。TBK1在活性环内Ser172位点的磷酸化对其激酶的活性是必需的，包括下游的IRF3的磷酸化(4,5)。

1. Tojima, Y. et al. (2000) Nature 404, 778-82.
2. Pomerantz, J.L. and Baltimore, D. (1999) EMBO J 18, 6694-704.
3. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
4. Kishore, N. et al. (2002) J Biol Chem 277, 13840-7.
5. McCoy, C.E. et al. (2008) J Biol Chem 283, 14277-85.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Xenopus, Bovine, Dog

Specificity / Sensitivity

Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb detects endogenous levels of TBK1 only when phosphorylated at Ser172.

Source / Purification

Monoclonal antibody is prepared from animals immunized with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1.

Background

TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1/NAK induces IκB degradation and NF-κB activity through IKKβ. NAK may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).TBK1 is phosphorylated at Ser172 within its activation loop, which is necessary of its kinase activity including the downstream phosphorylation of IRF3 (4,5).

1. Tojima, Y. et al. (2000) Nature 404, 778-82.
2. Pomerantz, J.L. and Baltimore, D. (1999) EMBO J 18, 6694-704.
3. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
4. Kishore, N. et al. (2002) J Biol Chem 277, 13840-7.
5. McCoy, C.E. et al. (2008) J Biol Chem 283, 14277-85.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.