Mouse Basic Fibroblast Growth Factor (mFGF basic/FGF2)

• 产品编号：CST-5414LC      品牌：CST       原厂货号：5414LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > FGF 成纤维细胞生长因子
• 应用分类：

描述：

Cell Signaling Technology公司生产的重组鼠FGF basic 蛋白（mFGF basic）Ala11-Ser154 (Accession #NP_032032)在大肠杆菌中表达。重组的mFGF basic氨基末端没有甲硫氨酸，计算得到的分子量为16,354.DTT-还原和非还原状态下蛋白迁移状态与17KDa多肽相同。重组mFGF basic氨基端序列通过氨基酸测序确认。重组mFGF basic使用NIH/3T3细胞扩增实验检验其生物活性。每个批次的ED50值都在50-400ng/ml。带载体： 0.33mg/ml mFGF basic溶于含有10 mM DTT 和20 μg BSA pH 7.2 的PBS溶液经0.22um滤器过滤获得。无载体：0.33mg/ml mFGF basic溶于含有10 mM DTT pH 7.2 的PBS溶液经0.22um滤器过滤获得。胚胎和成体细胞均能生成成纤维细胞因子(FGF basic，FGF2)，成纤维细胞因子与各种疾病的发生相关，包括癌症和动脉粥样硬化（1）。FGF2涉及发育过程并且参与调控分化，增殖和转移（1-6）。FGF2是在细胞培养中维持胚胎干细胞的生长而不分化的重要调控因子。FGF2与硫酸肝素有高亲和性（1,2），并且这一结合是FGF2激活FGFR赖氨酸激酶的其中一个步骤。有4个不同的FGF受体，每一个都有多个剪切异构体。FGF2与多个，但不是全部的FGFRs有高亲和性。FGF basic向FGFR的信号传递包括ras-raf-MAPK, PLCγ/PKC, 和PI3K/AKT信号通路（1）。

原厂资料：

Source / Purification

Recombinant mouse FGF basic (mFGF basic) Ala11-Ser154 (Accession #NP_032032) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mFGF basic does not have a Met on the amino terminus and has a calculated MW of 16,354. DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides. The expected amino terminal ALPED of recombinant mFGF basic was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mFGF basic. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mFGF basic was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 50-400 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mFGF basic.

Formulation

With carrier: A 0.22 μm filtered solution of 0.33 mg/ml mFGF basic in PBS, pH 7.2 containing 10 mM DTT and 20 μg BSA per 1 μg mFGF basic. Carrier free: A 0.22 μm filtered solution of 0.33 mg/ml mFGF basic in PBS, pH 7.2 containing 10 mM DTT.

Background

FGF basic (FGF2) is producted in both embryonic and adult cell types, and contributes to the pathogenesis of various diseases, including cancer and atherosclerosis (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and binding is a step in the FGF basic activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).

注意事项：

Storage: Stable in lyophilized state at 4°C for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4°C for 2 months and at -20°C for 6 months. Avoid
repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C
should be in a manual defrost freezer