Human Interleukin-29 (hIL-29)

• 产品编号：CST-5183LC      品牌：CST       原厂货号：5183LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司生产的人重组蛋白IL-29 (hIL-29) Gly20-Thr200 (Accession #NP_742152)是从人的293细胞表达而来。重组的hIL-29蛋白没有标签，未糖基化蛋白分子量据推算为20,018Da。DTT-还原的蛋白和未还原的蛋白作为31kDa的多肽而迁移。在SDS-PAGE中较低的迁移率是由于糖基化的作用。重组蛋白hIL-29的N-末端序列GPVPT通过测序而证实。6 μg 还原 (+) 和 非还原的(-) 重组hIL-29通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hIL-29的生物活性是通过病毒保护实验来确定的。每个批次的ED50在0.40-1.45 ng/ml之间。

有载体：每微克mIFN-γ蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mIFN-γ蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。IL-29是干扰素λ细胞因子家族成员之一，并参与到I型干扰素家族的功能中(1,2)。IL-29在单核细胞和树突细胞对病毒感染的应答过程中而产生并且刺激Toll样受体的配体(1-3)。IL-29是潜在的抗病毒的活性的细胞因子。IL-29的抗病毒活性包括上调I类MHC在细胞膜上的表达、PKR、MxA和 2’, 5'-OAS 的表达(1,2)。IL-29的受体由 IL-28Rα和IL-10Rβ组成并在多数非造血功能的细胞上表达 (3)。IL-29的结合诱导了 Stats 1, 2 和 3的磷酸化(1,3)。

原厂资料：

Source / Purification

Recombinant human IL-29 (hIL-29) Gly20-Thr200 (Accession #NP_742152) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-29 contains no "tags" and the nonglycosylated protein has a calculated MW of 20,018. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal GPVPT of recombinant hIL-29 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-29. All lots are greater than 98% pure.

Bioactivity

The bioactivity of hIL-29 was determined in a virus protection assay. The ED50 of each lot is between 0.40-1.45 ng/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-29. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-29 is a member of the IFNλ family of cytokines and shares many functions with the type I family of interferons (1,2). IL-29 is produced by monocytes and dendritic cells in response to viral infection and stimulation with toll-like receptor ligands (1-3). IL-29 is a potent antiviral cytokine. The antiviral activities of IL-29 include the upregulation of MHC Class I expression on the cell surface, and the expression of PKR, MxA, and 2’, 5'-OAS (1,2). The IL-29 receptor consists of IL-28Rα and IL-10Rβ is expressed on most non-hematopoietic cells (3). IL-29 binding induces the phosphorylation of Stats 1, 2 and 3 (1,3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles