HCK 10 UG

• 产品编号：PV6128      品牌：invitrogen       原厂货号：PV6128
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 蛋白激酶 > 丝氨酸/苏氨酸激酶
• 应用分类：

描述：

This recombinant fusion protein consists of full-length hematopoietic cell kinase (HCK) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
•  Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
•  Expressed in insect cells
• GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

HCK for the LanthaScreen Kinase Binding Assay
Hematopoietic cell kinase (HCK) is a SRC-related kinase expressed in lymphoid and hematopoietic cells and may play a role in some forms of leukemia. (HCK Alias: DKFZp686D0638)

原厂资料：

注意事项：

For Research Use Only. Not for use in diagnostic procedures.