IGF-1R/3T3 mouse fibroblasts transfected with human type 1 IGF-receptor cDNA.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.2 mg/ml
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage Conditions
4° C
Tested Applications
Dilution *
ELISA (ELISA)
Assay Dependent
Immunocytochemistry (ICC)
1:10-1:100
Immunofluorescence (IF)
Assay-Dependent
Immunoprecipitation (IP)
2µg per mg lysate
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with an epitope at aa 283-440 (exon 4-6).
Recommended positive controls include placenta or breast tissue. This antibody is not suitable for Western blot. Without BSA, this antibody can be used as a capture antibody in a sandwich ELISA.
Background/Target Information
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.