BUF058C is a unique aqueous-based mounting medium designed for the permanent preservation of tissue sections and cell smears which have been immunohistochemically stained with chromogens which cannot be dehydrated with organic solvents.
BUF058C is suitable for use with peroxidase and alkaline phosphatase chromogens, Fast red, BCIP/NBT, BCIP/INT, Aminoethylycarbazole (AEC) and DAB/DAB with cobalt and nickel chromogens, and is also compatible with Haematoxylin and Nuclear fast red (NFR) counterstains.
BUF058C is not suitable for use with Haematoxylin and Eosin (H and E) staining.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at room temperature (+25oC). For long term storage recommend 2-8oC. DO NOT FREEZE.
Shelf Life
18 months from date of despatch.
Instructions For Use
Ensure product is at room temperature before use.
1. Rinse slide for mounting with distilled or deionized water, touch-dry slide edges on paper towel and place slide on flat surface.
2. Invert vial and open dropper to remove air bubbles.
3. Apply 3-4 drops of mounting medium directly onto specimen and tilt slide back and forth to distribute evenly. A plastic pipette tip may also be used for spreading, taking care not to damage specimen. Remove excess medium by touching slide edges on paper towel.
4. Leave slide to stand for 1-2 hours at room temperature (25oC). Alternatively slide can be heated for 40-60 minutes at 37-40oC.
Mounted slide can now be visualized under a microscope:
1. For oil immersion lens, the oil can be dropped directly onto the mounted specimen, once the mounting medium has dried. A coverslip is not required. The oil can be later removed by wiping with Kim wipes.
OR
2. For oil immersion lens, apply 1-2 drops of preferred organic mounting medium on top of dried mounted specimen and cover with coverslip.
To remove coverslip or mounting medium, soak the slide for 5-10 minutes in warm water (37oC) until medium is dissolved. Give slide final rinse in warm water. Remount if required.
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