This AbD Serotec Four-Color Human Flow Kit can be used for single-step identification of activated helper/inducer (CD3+CD4+CD38+ and CD3+CD4+HLA DR+) T-cell subsets.
CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the 20kDa CD3ε chain.
CD4 is a single chain transmembrane glycoprotein and member of the immunoglobulin superfamily, which interacts directly with class II molecules of the major histocompatibility complex (MHCII), and is the primary receptor for the human immunodeficiency virus (HIV). CD4 is expressed by the helper/inducer (CD3+CD4+) T-cell subset, the majority of thymocytes, and at low levels by monocytes and tissue macrophages. The CD4 lymphocyte count provides an excellent indication of immune system health. Clone RPA-T4 binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen.
CD38, otherwise known as cyclic-ADP ribose hydrolase 1, is a type II integral transmembrane glycoprotein and member of the ADP-ribosyl cyclase family, which is widely used to study the processes of B- and T-cell differentiation and activation. Increased expression of CD38 on both CD4+ and CD8+ T-cells in HIV-infected patients is associated with disease progression.
HLA DR is a heterodimeric cell surface glycoprotein and human class II MHC (major histocompatibility complex) cell surface receptor, consisting of a 36kDa alpha and 27kDa beta chain, which is essential for efficient peptide presentation to CD4+ T-cells. HLA DR is expressed primarily by antigen presenting cells and, together with CD38, is a useful marker of T-cell activation following viral infection. Clone YE2/36-HLK recognises a monomorphic determinant of human HLA DR.
Product Form
Four color combination consisting of APC, FITC, RPE-Cy5.5 and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
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