Our Amplite™ Fluorimetric Acetylcholinesterase Assay Kit provides one of the most sensitive methods for detecting AChE activity or screening AChE inhibitors in red florescence window. The kit uses Amplite™ Red to quantify the choline produced from the hydrolysis of acetylcholine by AChE through choline oxidase-mediated enzyme coupling reactions. It can be used for monitoring and quantifying the AChE activity in blood, cell extracts or other solutions. The fluorescence intensity of Amplite™ Red is used to measure the amount of choline formed, which is proportional to the AChE activity. The kit is an optimized “mix and read” assay that provides a simple one-step fluorimetric assay to detect as little as 0.01 mU AChE in a 100 μL assay volume (0.1 mU/mL). Its signal can be easily read with a fluorescence microplate reader at Ex/Em = ~540/590 nm or an absorbance microplate reader at ~575 nm.
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