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Amplite™ Fluorimetric Catalase Assay Kit *Red Fluorescence*

 
包装: 1 kit
运保温度: Keep in freezer Avoid exposure to light
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其它组分:

Amplite™ Red【包装:1 vial】
H2O2【包装:1 vial (3% stabilized solution, 200 μL)】
Assay Buffer【包装:1 bottle (20 mL)】
Horseradish Peroxidase【包装:1 vial (20 units)】
Catalase Standard【包装:1 vial (1000 U/mL, 50uL)】
DMSO【包装:1 vial (200 μL)】


描述:

The Amplite. Fluorimetric Catalase Assay Kit provides a quick and sensitive method for the measurement of catalase activity. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Catalase reacts with H2O2 to produce water and oxygen (O2). Amplite. Red also reacts with H2O2 to generate a red fluorescent product. Therefore the reduction in fluorescence intensity is proportional to catalase activity. The Amplite. Red substrate used in the assay enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm. With the Amplite. Fluorimetric Catalase Assay Kit, we have detected as little as 30 mU/mL catalase in a 100 μL reaction volume.


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