READY ONE-STEP RT-PCR KITREADY ONE-STEP RT-PCR KIT

• 产品编号：Amre-1B1326-50RXN      品牌：Amresco       原厂货号：1B1326-50RXN
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > RT-PCR（Endpoint）
• 应用分类：

描述：

Overview
•One-step cDNA synthesis
•Load directly to gel
•Convenient format
•Immediate visualization after electrophoresis
•Safe alternative to ethidium bromide

Detailed Description
Ready One-Step RT-PCR Kit offers a single step procedure for performing RT-PCR reactions. Subsequent loading and visualization of RT-PCR products are streamlined since the loading buffer and visualization dye are included. The user supplies the primers and the DNase-treated template RNA.

Ready One-Step RT-PCR Kit includes Ready One-Step Reverse Transcriptase and Ready One-Step RT-PCR Mix, 2X. Ready One-Step Reverse Transcriptase consists of M-MLV and a proprietary buffer that delivers robust cDNA synthesis from rare and abundant transcripts. Ready One-Step RT-PCR Mix, 2X consists of a mixture of reaction buffer, AMRESCO's Extender Taq polymerase blend, dNTPs, an electrophoresis tracking dye, and a non-mutagenic EZ-Vision visualization dye. Once amplification is complete, the PCR reaction can be directly loaded and separated on an agarose gel using the magenta-colored tracking dye (migrating at approximately 10 bp on a 1% gel). After electrophoresis the PCR products are immediately visualized with standard UV illumination without additional post-run staining and destaining steps. Available kits provide sufficient material for 50 or 100 reactions.

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