Thermo ScientificPfuDNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeumPyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction.PfuDNA Polymerase also exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’ exonuclease activity.
Generation of PCR products for cloning and expression
RT-PCR for cDNA cloning and expression
Blunt-end PCR cloning
Includes
PfuDNA Polymerase (native or recombinant, 2.5 U/µL)
10XPfuBuffer with MgSO4
10XPfuBuffer
25 mM MgSO4
原厂资料：
Thermo ScientificPfuDNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeumPyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction.PfuDNA Polymerase also exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’ exonuclease activity.
Generation of PCR products for cloning and expression
RT-PCR for cDNA cloning and expression
Blunt-end PCR cloning
Includes
PfuDNA Polymerase (native or recombinant, 2.5 U/µL)
10XPfuBuffer with MgSO4
10XPfuBuffer
25 mM MgSO4
注意事项：
The error rate ofPfuDNA Polymerase in PCR is 2.6 x 10-6errors per nt per cycle as determined by a modified method described by Lundberget al. Accordingly, the accuracy of PCR is 3.8 x 105. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs. ThePfuDNA Polymerase has no detectable reverse transcriptase activity. dUTP, dITP, and primers containing these nucleotides should not be used in PCR withPfuDNA Polymerase because the binding of this enzyme to DNA templates with uracil and hypoxanthine stalls DNA synthesis.
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