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T7 Express Competent E. coli (High Efficiency)

 
包装:
运保温度: -80°C
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描述:

• Transformation efficiency: 0.6-1 x 109 cfu/μg pUC19

• Enhanced BL21 derivative

• T7 RNA Polymerase in the lac operon - no λ prophage

• Deficient in proteases Lon and OmpT

• Resistant to phage T1 (fhuA2)

• Does not restrict methylated DNA (McrA-, McrBC-, EcoBr-m-, Mrr-)

• B Strain

• Free of animal products

Description:
Chemically competent E. coli cells suitable for high efficiency transformation and protein expression.

Reagents Supplied:
C2566H:
20 x 0.05 ml/tube of chemically competent T7 Express Competent E. coli cells (Store at -80°C)
20 ml of SOC outgrowth medium (Store at room temperature)
0.025 ml of 50 pg/µl pUC19 Control DNA (Store at -20°C)

 


注意事项:

General notes:

  1. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
  2. STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.


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