• Transformation efficiency: 0.6-1 x 109 cfu/μg pUC19
• Enhanced BL21 derivative
• T7 RNA Polymerase in the lac operon - no λ prophage
• Deficient in proteases Lon and OmpT
• Resistant to phage T1 (fhuA2)
• Does not restrict methylated DNA (McrA-, McrBC-, EcoBr-m-, Mrr-)
• B Strain
• Free of animal products
Description: Chemically competent E. coli cells suitable for high efficiency transformation and protein expression.
Reagents Supplied: C2566H:
20 x 0.05 ml/tube of chemically competent T7 Express Competent E. coli cells (Store at -80°C)
20 ml of SOC outgrowth medium (Store at room temperature)
0.025 ml of 50 pg/µl pUC19 Control DNA (Store at -20°C)
原厂资料:
注意事项:
General notes:
CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.