CLIP-tag Purified Protein

• 产品编号：P9311S      品牌：New England Biolabs       原厂货号：P9311S
• 产品分类：生化和细胞分析 > 标签、标记试剂盒 > 细胞标记试剂及试剂盒
• 应用分类：

描述：

CLIP-tag™ is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a small protein tag based on human O⁶-alkylguanine-DNA-alkyltransferase (AGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether link. Although CLIP-tag is based on the same protein as SNAP-tag^®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal simultaneous labeling.

CLIP-tag Purified Protein can be used as a positive control for in vitro labeling with various CLIP-tag fluorescent substrates. The coding sequence of CLIP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. CLIP-tag protein (MW: 20,675) was expressed and purified according to the instructions in the IMPACT™ kit manual (NEB #E6901). The purified CLIP-tag protein was dialyzed into 1X phosphate buffered saline (PBS) solution containing 1 mM DTT at 1 mg/ml (50 μM) and stored at -80°C.

原厂资料：

注意事项：

Storage: The CLIP-tag Purified Protein may be stored at -20°C for short term use, but should be kept at -80°C for long term storage. CLIP-tag Purified Protein is supplied at a concentration of 50 μM in 1X PBS with 1 mM DTT. Thaw and aliquot just before use.