RNase B

产品编号：P7817S 品牌：New England Biolabs 原厂货号：P7817S
产品分类：克隆 > DNA/RNA修饰酶 > RNase
应用分类：

包装：

运保温度:

-20°C

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

RNase B is a high mannose glycoprotein (1-3) that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates. RNase B has a single N-linked glycosylation site which makes it ideal for SDS-PAGE gel shift assays.

Product Source

Bovine pancreas

Storage Conditions

50 μl Milli-Q water
pH 7.0 @ 25°C

Molecular Weights

Theoretical: 17000 daltons

注意事项：

250 μg is enough for approximately 20 reactions.
RNase B Deglycosylation Protocol:
1. In a 10 µl reaction, add 2 µl of RNase B, 1 µl of 10X Glycoprotein Denaturing Buffer, and 5 µl of dH₂O.
2. Boil for 10 minutes.
3. Add 1 µl of 10X G7 Reaction Buffer and 1 µl of 10% NP-40.
4. Add 1 µl of endoglycosidase. Incubate at 37°C for 1 hour.
5. Visualize reaction products on 10–20% SDS-PAGE gel.