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Quick-Load PCR DNA Ladder

 
包装: 125 gel lanes
运保温度: 4°C
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描述:

Description: 
Quick-Load® PCR Marker is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

The Quick-Load PCR Marker consists of a proprietary plasmid which is digested to completion with appropriate restriction enzymes to yield 5 double-stranded DNA bands that are suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 50–766 base pairs.
Effective Size Range: 50 bp to 766 bp

Concentration:
30 μg/ml

Recommended Load: 0.3 μg

Storage Conditions:
3.3 mM Tris-HCl
11 mM EDTA
0.015% Bromophenol Blue
0.017% SDS
2.5% Ficoll 400
pH 8.0 @ 25°C


注意事项:

General notes:

Quick-Load PCR Marker is stable for at least 6 months at 25°C.

For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

Usage notes:

Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.


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